Okada Y. (Ed.) Patch Clamp Techniques: From Beginning to Advanced Protocols

Springer, 2012. — 437 p.
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.Prologue: The Ion Channel
Patch-Clamp Techniques: General Remarks
Whole-Cell Patch Method
Perforated Whole-Cell Patch-Clamp Technique: A User’s Guide
Methods for Processing and Analyzing Single-Channel Data
Channel Noise
Slice Patch Clamp
Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms
Analysis of Synaptic Plasticity with the Slice Patch-Clamp Recording Technique
Patch-Clamp Recordings from Neuronal Dendrites
In Vivo Blind Patch-Clamp Recording Technique
Two-Photon Targeted Patch-Clamp Recordings in Vivo
Transporter Current Measurements
Giant Patch and Macro Patch
Pipette Perfusion Technique
Planar Lipid Bilayer Method for Studying Channel Molecules
Patch-Clamp Capacitance Measurements
Brief Guide to Patch-Clamp Current Measurements in Organelle Membranes
Role of Ion Channels in Plants
Automated Patch Clamping
Patch-Clamp Biosensor Method
Temperature-Evoked Channel Activation: Simultaneous Detection of Ionic Currents and Temperature
Heterologous Expression Systems and Analyses of Ion Channels
Patch-Clamp and Single-Cell Reverse Transcription-Polymerase Chain Reaction/Microarray Analysis
Smart-Patch Technique
Ion Channel Pore Sizing in Patch-Clamp Experiments
Digital Recordings of Patch-Clamp Data
Solutions for Patch-Clamp Experiments