Darzynkiewicz Z., Holden E., Orfao A., Telford W., Wlodkowic D. (eds.) Recent Advances in Cytometry, Part B: Advances in Applications

5th Edition. - Elsevier, 2011. - 385 p. -(Methods in Cell Biology, Volume 103) Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay.
* Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays
* Discusses many new assays developed since the previous edition
* Presents recent developments in cytometric intrumentation/technologyContents
New Applications in Cell Biology
Recent Advances in Cytometry Applications: Pre-clinical, Clinical, and Cell Biology
Detection of Hematopoietic Stem Cells by Flow Cytometry
Identification of very small embryonic/epiblast like stem cells (vsels) circulating in peripheral blood during organ/tissue injuries
Apoptosis and beyond: cytometry in studies of programmed cell death
Assessment of Oxidative Stress-Induced DNA damage by Immunoflourescent Analysis of 8-oxodG
Analysis of Individual Molecular Events of DNA Damage Response by Flow and Image Assisted Cytometry
Fluorescence-based detection and quantification of features of cellular senescence
Measurement of Telomere Length using PNA Probe by Cytometry
Pre-Clinical and Clinical Applications
Cytometry of Intracellular Signaling; From Laboratory Bench to Clinical Application
Immunophenotypic pattern of myeloid populations by flow cytometry analysis
Flow cytometry-based pharmacodynamic monitoring after organ transplantation
Clinical Cytometry and Progress in HLA Antibody Detection
Clinical Utility of Flow Cytometry in the Study of Erythropoiesis and Non-Clonal Red Cell Disorders
Immunophenotypic characterization of bone marrow MCs in mastocytosis and other mast cell disorders