Primrose S.B. Principles and Techniques of Biochemistry and Molecular Biology

7th ed. ,2006 - 667 p.
BLACKWELL PUBLISHING.
Main Street, Malden, MA 02148-5020, USA.
Garsington Road, Oxford OX4 2DQ, UK.
Swanston Street, Carlton, Victoria 3053, Australia.
The book describes the main technologies used in molecular genetics, genetic engineering and bioinformatics.
Gene manipulation in the post-genomics era.
Fundamental Techniques of Gene.
Manipulation.
Basic techniques.
Cutting and joining DNA molecules.
Basic biology of plasmid and phage vectors.
Cosmids, phasmids, and other advanced vectors.
Gene-cloning strategies.
Sequencing genes.
Changing genes: site-directed mutagenesis and protein engineering.
Bioinformatics.
Manipulating DNA in Microbes,Plants, and Animals.
Cloning in bacteria.
Cloning in Saccharomyces cerevisiae.
Gene transfer to animal cells.
Genetic manipulation of animals.
Gene transfer to plants.
Advanced transgenic technology.
Genome Analysis, Genomics, and Beyond.
The organization and structure of the genomes.
Mapping and sequencing genomes.
Comparative genomics.
Large-scale mutagenesis and interference.
Analysis of the transcriptome.
Proteomics I – Expression analysis and characterization of proteins.
Proteomics II – Analysis of protein structures.
Metabolomics and global biochemical networks.
produced commercially in bacteria, yeast,
Applications of Gene and Manipulation and Genomics.
Applications of genomics.
Applications of recombinant DNA technology.