Springer, 2010. — 150 p. — ISBN: 978-3-642-04608-7
This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Antibodies for Immunohistochemistry
Antibody Labeling and the Choice of the Label
Probes Processing in Immunohistochemistry
Working with Antibodies
Background Staining, Autofluorescence and Blocking Steps
Immunostaining Enhancement
Multiple Multicolor Immunoenzyme Staining
Multiple Immunofluorescence Staining
Antigen Detection on Tissues Using Primary Antibody Raised in the Same Species
Probes for Staining Specific Cellular Organelles
The Use of Epitope Tags in Histochemistry
Immunohistochemistry at the Ultrastructural Level
Diagnostic Immunohistochemistry
A Picture Is Worth a Thousand Words