Brown T.A. Genomes 4 (Part II)

4th Edition. — Garland Science, 2017. — 540 p. — ISBN: 978-0815345084
Genomes 4 has been completely revised and updated. It is a thoroughly modern textbook about genomes and how they are investigated. As with Genomes 3, techniques come first, then genome anatomies, followed by genome function, and finally genome evolution. The genomes of all types of organism are covered: viruses, bacteria, fungi, plants, and animals including humans and other hominids.
Genome sequencing and assembly methods have been thoroughly revised including a survey of four genome projects: human, Neanderthal, giant panda, and barley. Coverage of genome annotation emphasizes genome-wide RNA mapping, with CRISPR-Cas 9 and GWAS methods of determining gene function covered. The knowledge gained from these techniques forms the basis of the three chapters that describe the three main types of genomes: eukaryotic, prokaryotic (including eukaryotic organelles), and viral (including mobile genetic elements). Coverage of genome expression and replication is truly genomic, concentrating on the genome-wide implications of DNA packaging, epigenome modifications, DNA-binding proteins, non-coding RNAs, regulatory genome sequences, and protein-protein interactions. Also included are applications of transcriptome analysis, metabolomics, and systems biology. The final chapter is on genome evolution, focusing on the evolution of the epigenome, using genomics to study human evolution, and using population genomics to advance plant breeding. Established methods of molecular biology are included if they are still relevant today and there is always an explanation as to why the method is still important.
How genomes are studied
Genomes, Transcriptomes and Proteomes
Studying DNA
Mapping Genomes
Sequencing Genomes
Genome Annotation
Identifying Gene Functions
Genome anatomies
Eukaryotic Nuclear Genomes
Genomes of Prokaryotes and Eukaryotic Organelles
Virus Genomes and Mobile Genetic Elements
How genomes are expressed
Accessing the Genome
The Role of DNA-Protein Interactions in Genome Expression
Transcriptomes
Proteomes
Genome Expression in the Context of Cell and Organism
How genomes are replicated and evolve
Genome Replication
Mutations and DNA Repair
Recombination and Transposition
How Genomes Evolve