Gizeli E., Lowe C.R. (Eds.) Biomolecular Sensors

Taylor & Francis Inc, 2002. — 337 p. — ISBN: 074840791X.The development of devices that incorporate biological assemblies is impacting analytical and biomedical research. Today, scientists can monitor vital biological interactions-such as the binding of DNA to proteins-in real time, deriving unique information necessary to understanding biochemical pathways and thus aiding the design of drugs to regulate these processes. Biomolecular Sensors describes the development of this new technology covering the fundamental physics through to applications. The principles of biological recognition, immobilization techniques, and transducer technology are described in depth in order to provide the necessary background to readers from various disciplines. Focusing on the study of antigen-antibody, DNA-protein and receptor-ligand binding events, the book covers biological applications and describes the commercial instruments available to exploit this technology in areas as diverse as biological research, medical applications and environmental monitoring. With contributions from internationally recognized experts, this is the essential reference for advanced undergraduates and postgraduates as well as professionals in this rapidly advancing field.Biological recognition
Principles of antigen–antibody recognition
Protein–protein interactions
DNA interactions
Immobilisation of biomolecules
Immobilisation chemistry of biological recognition molecules
Binding isotherms and kinetics of immobilized biological systems
Transducer technology
Optical transducers
Acoustic transducers
Immunoassays using enzymatic amplification electrodes
Applications
Surface plasmon resonance: development and use of BIACORE instruments for biomolecular interaction analysis
IAsys: the resonant mirror biosensor
Commercial quartz crystal microbalances: theory and applications
The quartz crystal microbalance with dissipation monitoring (QCM-D)