Saltzman Mark W., Shen H., Brandsma Janet L. DNA Vaccines Methods and Protocols

Humana Press, 2006. — 400 p. — ISBN10: 1588294846; ISBN13: 978-1588294845.In this completely revised and updated second edition of DNA Vaccines: Methods and Prools, W. Mark Saltzman presents a comprehensive collection of DNA vaccine prools, written by leading experts in the groundbreaking field of DNA vaccination. Divided into five sections, this volume contains state-of-the-art and practical procedures on the latest DNA vaccine technology. Part I contains DNA vaccine design prools, focusing on methods that achieve optimal expression in host cells. Part II is dedicated to presenting methods for DNA delivery, and covers both the range of administration methods available for vaccine administration and a variety of techniques for improving the efficiency of delivery into cells. Part III discusses current available methods, including adjuvant and prime-boost approaches, for enhancing the potency of DNA vaccines. Part IV describes several key areas of application in the field, including allergy, avoidance of autoimmunity, and neonate and infant vaccine response. DNA Vaccines: Methods and Prools, Second Edition concludes with a review of prools for vaccine production and purification, and applicable quality control methods.DNA Vaccine Design.
DNA Vaccine Design.
Design of Plasmid DNA Constructs for Vaccines.
Vaccination with Messenger RNA.
A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines.
In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors.
DNA Vaccine Delivery Systems.
Delivery of DNA Vaccines Using Electroporation.
Needle-Free Injection of DNA Vaccines.
Needle-Free Delivery of Veterinary DNA Vaccines.
Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery.
A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Non-Viral Hybrid Approach.
Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence.
DNA Vaccine Adjuvants and Activity Enhancement.
Adjuvant Properties of CpG Oligonucleotides in Primates.
Complexes of DNA Vaccines with Cationic, Antigenic Peptides are Potent, Polyvalent CD8+ T Cell-Stimulating Agents.
Prime Boost Strategies in DNA Vaccines.
Modifying Professional Antigen Presenting Cells to Enhance DNA Vaccine Potency.
Replicase-Based DNA Vaccines for Allergy Treatment.
DNA Vaccine Applications.
Immunological Responses of Neonates and Infants to DNA Vaccines.
DNA Vaccines for Allergy Treatment.
Protection From Autoimmunity by DNA Vaccination Against T Cell Receptor (TCR).
Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms.
DNA Vaccine Production, Purification, and Quality.
A Simple Method for the Production of Plasmid DNA in Bioreactors.
Practical Methods for Supercoiled pDNA Production.
Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines.
Large-Scale Non-Chromatographic Purification of Plasmid DNA.
Assuring the Quality, Safety and Efficacy of DNA Vaccines.