Thomas D., Jessop Z., Whitaker I. (Eds.) 3D Bioprinting for Reconstructive Surgery: Techniques and Applications

Woodhead Publishing, 2018. — 452 p. — (Woodhead Publishing Series in Biomaterials). — ISBN: 978-0-08-101103-4.This book examines the combined use of materials, procedures and tools necessary for creating structural tissue constructs for reconstructive purposes. Offering a broad analysis of the field, the first set of chapters review the range of biomaterials which can be used to create 3D-printed tissue constructs. Part Two looks at the techniques needed to prepare biomaterials and biological materials for 3D printing, while the final set of chapters examines application-specific examples of tissues formed from 3D printed biomaterials.3D printing of biomaterials for tissue engineering applications is becoming increasingly popular due to its ability to offer unique, patient-specific parts — on demand — at a relatively low cost. This book is a valuable resource for biomaterials scientists, biomedical engineers, practitioners and students wishing to broaden their knowledge in the allied field.Introduction: Inception, evolution and future of 3D bioprinting
3D Bioprinting Techniques
Practical laboratory methods for 3D bioprintingOverview of 3D bioprinting methods
Biomaterials for printing
Owning a 3D bioprinter
Typical 3D bioprinting processes
Trends of bioprintingFurther readingComputational design of biostructuresEssential steps in the computational design of biostructures
Medical imaging
File formats
Image processing and 3D reconstruction
CAD
An overview of additive manufacture and 3D printing
Postprocessing
Application case studies
Case study 3: Tumor removal and custom craniofacial implant
DiscussionCell preparation for 3D bioprintingCell expansion
Sterility assessment
Cell phenotype characterization
Metabolic assessment3D bioprinting for scaffold fabricationDP for engineering scaffold
Transduction and bioprinting scaffold for tissue regeneration
Future directions and concluding remarksBiopolymer hydrogel bioinks
Bioprinting versus 3D printing and conventional cell seeding
Advantages and disadvantages of biopolymers for bioprinting
Biopolymer hydrogels
How to print macroscopic, stable structures with hydrogelsFurther readingSynthetic material bioinksBioink properties
Synthetic polymersCandidate bioinks for 3D bioprinting soft tissueBioink construct types
Bioink constituents
Determinant bioink moduli
Clinical application and selection
Future potentialAssessing printability of bioinksThe biofabrication window and printability
Nozzle/printing parameters and filament dimensions
Viscosity and rheology
Cross-linking and sacrificial/support materials
Computer simulation and modeling
Challenges and future outlookBioreactor processes for maturation of 3D bioprinted tissueProcess parameters
Bioreactor types
Building a perfusion bioreactor system for 3D tissue culture
Hydrostatic pressure analysis
Fluid-induced shear stress analysis
Discussion3D bioprinting: Regulation, innovation, and patentsRegulation
Patents as an actor for regulation
Conclusion and future trends
Sources of further informationFurther reading3D Bioprinting Applications
The clinical need for 3D printed tissue in reconstructive surgery
Bioprinting principles
Bioprinting for tissue assembly
Connective tissue reconstruction and the role of bioprinting3D bioprinting boneThe clinical need for bioprinted bone and limitations of current treatments
Biology, structure and functions of bone
Approaches to bone bioengineering
D bioprinting of bone tissue3D bioprinting cartilageLimitations of current tissue-engineered cartilage
Cartilage development
Understanding cartilage morphology
Combining strategies to tissue-engineer durable cartilage
Summary and future perspectivesFurther reading3D bioprinting adipose tissue for breast reconstructionD bioprinting
D bioprinting adipose tissue for breast reconstructionFurther reading3D bioprinting nerve
Advantage of 3D vs. 2D neural cell culture by implementing 3D printing technique
D-printing of biomedical materials followed by seeding with cells
D printing of biomedical materials with cells (bioprinting)
Summary and future perspectivesFurther reading3D bioprinting skinTechnical progress in 3D-bioprinted skin constructs
Cell type
Biomaterials
Bioprinter and bioprinting process
Design of skin constructs
Regeneration of skin appendages
Prospects of researches on skin substitutes3D bioprinting blood vesselsD bioprinting strategies
Sacrificial bioprinting for vascular engineering
Embedded bioprinting for vascular engineering
Hollow tube bioprinting for vascular engineering
Scaffold-free multicellular spheroid bioprinting for vascular engineering3D bioprinting composite tissueCharacteristics of composite tissues
Strategies for tissue fabrication
Future worksThe commercial 3D bioprinting industryThe market
Key companies
Translational steps
Future development