Popot Jean-Luc (ed.) Membrane Proteins in Aqueous Solutions: From Detergents to Amphipols

Springer, 2018. — 724 p. — (Biological and Medical Physics, Biomedical Engineering).This book is the first to be entirely devoted to the challenging art of handling membrane proteins out of their natural environment, a key process in biological and pharmaceutical research, but one plagued with difficulties and pitfalls. Written by one of the foremost experts in the field, Membrane Proteins in Aqueous Solutions is accessible to any member of a membrane biology laboratory. After presenting the structure, functions, dynamics, synthesis, natural environment and lipid interactions of membrane proteins, the author discusses the principles of extracting them with detergents, the mechanisms of detergent-induced destabilization, countermeasures, and recent progress in developing detergents with weaker denaturing properties. Non-conventional alternatives to detergents, including bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants and amphipols, are described, and their relative advantages and drawbacks are compared. The synthesis and solution properties of the various types of amphipols are presented, as well as the formation and properties of membrane protein/amphipol complexes and the transfer of amphipol-trapped proteins to detergents, nanodiscs, lipidic mesophases, or living cells. The final chapters of the book deal with applications: membrane protein in vitro folding and cell-free expression, solution studies, NMR, crystallography, electron microscopy, mass spectrometry, amphipol-mediated immobilization of membrane proteins, and biomedical applications.Important features of the book include introductory sections describing foundations as well as the state-of-the-art for each of the biophysical techniques discussed, and topical tables which organize a widely dispersed literature. Boxes and annexes throughout the book explain technical aspects, and twelve detailed experimental protocols, ranging from in vitro folding of membrane proteins to single-particle electron cryomicroscopy, have been contributed by and commented on by experienced users.Membrane Proteins in Aqueous Solutions offers a concise, accessible introduction to membrane protein biochemistry and biophysics, as well as comprehensive coverage of the properties and uses of conventional and non-conventional surfactants. It will be useful both in basic and applied research laboratories and as a teaching aid for students, instructors, researchers, and professionals within the field.Membrane Proteins and Their Natural Environment
Extracting Membrane Proteins from Their Native Environment
Alternatives to Detergents for Handling Membrane Proteins in Aqueous Solutions
Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols
Formation and Properties of Membrane Protein/Amphipol Complexes
Amphipol-Assisted Folding of Membrane Proteins
Amphipol-Assisted Cell-Free Expression of Membrane Proteins
Optical Spectroscopy of Membrane Protein/Amphipol Complexes
Solution Studies of Membrane Protein/Amphipol Complexes
Nuclear Magnetic Resonance Studies of Amphipol-Trapped Membrane Proteins
Amphipols and Membrane Protein Crystallization
The Use of Amphipols for Electron Microscopy
Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications
The Use of Amphipols in Mass Spectrometry
Biomedical Applications