Nadeau J.L. Introduction to Experimental Biophysics. Biological Methods for Physical Scientists

2nd edition. — Boca Raton: CRC Press, 2017. — 791 p.Provides a complete introduction to molecular biology techniques, including optional advanced techniques and a laboratory manual for experimental practice.
Includes more chapter problems and questions incorporated for testing and self-study.
Brand new chapters on gene expression techniques (microarrays, mRNA and RNA interference), and advanced techniques in biological light microscopy (two-photon microscopy to fluorescence lifetime imaging microscopy and super-resolution techniques).
Additional chapters on holography and gold nanoparticles used in medicine.
This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions.Gives full color presentation for several figures with an expanded glossary, updated references and supplier list.