An Yuehuei H., Martin Kylie L. (eds.) Handbook of Histology Methods for Bone and Cartilage

Humana Press, 2003. — 582 p.Histologists and histochemists must spend considerable effort today to find the methods essential to perform bone and cartilage-related research. In Handbook of Histology Methods for Bone and Cartilage, Y.H. An and K.L. Martin have assembled an extensive collection of the major methods for studying the histology and histomorphometry of bone and cartilage. Described by well-experienced researchers, physicians, and technicians, the methods include techniques for bone labeling, bone sectioning, histological analysis, histomorphometric analysis, radiological examination, and contact radiography. Among the applications presented are in situ hybridization of bone and cartilage, radiological examination of calcified tissue with emphasis on bone, and dealing with histology artifacts in hard-tissue research. There are also histological techniques for the analysis of bone-implant interfaces, cartilage-defect repair, and the soft-tissue biomaterial interface. More practical than theoretical, the text is simple and straightforward, with many tables, line drawings, and micro- and macro photographs to illustrate the content. Full bibliographies at the end of each chapter guide the reader to additional detailed information.
Comprehensive and up-to-date, the Handbook of Histology Methods for Bone and Cartilage offers researchers a practical guide to all the major histological and histomorphometrical methods of bone and cartilage analysis-a gold mine of detailed techniques for the preparation of tissue from collection through fixation, embedding, sectioning, and staining.Introduction to Experimental Bone and Cartilage Histology
Cell Structure and Biology of Bone and Cartilage
Normal Structure and Function of Bone
Structure and Function of Articular Cartilage
Bone-Labeling Techniques
Human Bone Biopsy
Biopsy Issues for Bone and Cartilage Tumors
Tissue Harvesting and Fixation
Common Fixatives In Hard-Tissue Histology
Decalcification of Bone Tissue
Principles of Embedding and Common Protocols
Infiltration Techniques and Results in Different Types of Resin
Histological Techniques for Decalcified Bone and Cartilage
Techniques for Sectioning Undecalcified Bone Tissue Using Microtomes
Cutting and Grinding Methods for Hard-Tissue Histology
Bone Sectioning Using the Exakt System
Bone Sectioning Using a Modified Inner Diamond Saw
Bone Sectioning Using the Precise 1 Automated Cutting System
Bone, Cement, and Metal Implant Interface Preparation with a High-Pressure Water Cutter
Basic Staining and Histochemical Techniques and Immunohistochemical Localizations Using Bone Sections
Basic Staining Techniques for Cartilage Sections
Histochemical and Immunohistochemical Staining of Cartilage Sections
Staining Techniques for Plastic-Embedded Specimens
In Situ Hybridization of Bone and Cartilage
Bone Histomorphometry