Kaestner L. Calcium signalling: Approaches and Findings in the Heart and Blood

Berlin: Springer, 2013. — 246 p.The book combines general concepts and methods to investigate calcium signalling in cells ranging from molecular biology approaches to manipulation of calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in particular on optical sectioning techniques and fast image acquisition. In addition to these general guidelines there are application examples in a context beyond calcium signalling in two major fields: investigations of isolated cardiac myocytes and red blood cell related research. While the cellular cardiology section provides snapshots of certain calcium signalling aspects, the red blood cell part presents an overview from the functional identification of calcium-channels to a concept of physiological and pathophysiological relevance.Direct evidence - the digital approach
Fluorescence-based visualisation
Aequorin-based measurements
Measurement of calcium transport across membranes
Molecular biology based approaches
Manipulation of calcium
Calcium-induced function
Calcium signalling in cardiac myocytes
Calcium signalling in red blood cells
PerspectiveNon-linear and ultra high-speed imaging for explorations of the murine and human heart
A system for optical high resolution screening of electrical excitable cells
Concepts for optical high content screens of excitable primary isolated cells
A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventricular myocytes
Calcium imaging of individual erythrocytes: Problems and approachesOverexpression of junctin causes adaptive changes in cardiac myocyte Ca 2+ signaling
Remodelling of Ca 2+ handling organelles in adult rat ventricular myocytes during longterm culture
Functional and morphological preservation of adult ventricular myocytes in culture by sub-micromolar cytochalasin D supplementThe non-selective voltage-activated cation channel in the human red blood cell membrane: reconciliation between two conflicting reports and further characterisation
Ion channels in the human red blood cell membrane: Their further investigation and physiological relevance
Prostaglandin E 2 activates channelmediated calcium entry in human erythrocytes: An indication for a blood clot formation supporting process
Functional NMDA receptors in rat erythrocytes
Stimulation of human red blood cells leads to Ca 2+ -mediated intercellular adhesion
Lysophospatidic acid induced red blood cell aggregation in vitro
Regulation of phosphatidylserine exposure in red blood cells
Cation Channels in Erythrocytes - Historical and Future Perspective