Muller-Reichert Th. (Ed.) Electron Microscopy of Model Systems

Academic Press, Elsevier, 2010, 306 p.This volume covers the preparation and analysis of model systems for biological electron microscopy.This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems.
Covers the preparation and analysis of model systems for biological electron microscopy. Includes the most popular systems but also organisms that are less frequently used in cell biology. This issue presents the currently most important methods for the preparation of biological specimens. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems.
Finally, this book is also a reflection of an ongoing discussion in the field of biological electron microscopy. What is the best method of fixation? Some authors argue for the exclusive application of cryopreparation and imaging, while others emphasize the need for initial chemical fixation, or the requirement for inactivation of infectious material. Again, a decision for any of the methods presented here strongly depends on the biological question asked, the size of the biological system, and the practicality of the approach. The list of model systems presented here is by no means complete, but it is hoped that the models and techniques that are represented will help the reader to find appropriate methods for the preparation of her/his favorite system for electron microscopy.Preface/Acknowledgements
Electron microscopy of viruses
Bacterial TEM: New insights from cryo-microscopy
Analysis of the ultrastructure of archaea by electron microscopy
Chlamydomonas: Cryopreparation methods for the 3-D analysis of cellular organelles
Ultrastructure of the asexual blood stages of Plasmodium falciparum
Electron tomography and immunolabeling of Tetrahymena thermophila basal bodies
Electron microscopy of Paramecium
Ultrastructural investigation methods for Trypanosoma brucei
Dictyostelium discoideum: A model system for ultrastructural analyses of cell motility and development
Towards sub-second correlative live-cell light and electron microscopy of Saccharomyces cerevisiae
Fission yeast: A cellular model well suited for electron microscopic investigations
High-pressure freezing and electron microscopy of Arabidopsis
Standard and cryo-preparation of Hydra for transmission electron microscopy
Electron microscopy of flatworms: Standard and cryopreparation methods
Three-dimensional reconstruction methods for C. elegans ultrastructure
Insects as model systems in cell biology
Electron microscopy of amphibian model systems (Xenopus laevis and Ambystoma mexicanum)
Modern approaches for ultrastructural analysis of the zebrafish embryo
Transmission electron microscopy of cartilage and bone
Electron microscopy of the mouse central nervous system
Rapidly excised and cryofixed rat tissue
The actin cytoskeleton in whole mounts and sections
Three-dimensional cryo-electron microscopy on intermediate filaments: A critical comparison to other structural methods
Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cells
Viral infection of cells in culture: Approaches for electron microscopy
Intracellular membrane traffic at high resolution
3D versus 2D cell culture: Implications for electron microscopy
‘Tips and tricks’ for high-pressure freezing of model systems