12th Edition. — Elsevier, 2017. — 595 p. — ISBN: 978-0-7020-6696-2.
For more than 65 years, this best-selling text by Drs. Barbara J. Bain, Imelda Bates, and Mike A. Laffan has been the worldwide standard in laboratory haematology. The 12th Edition of Dacie and Lewis Practical Haematology continues the tradition of excellence with thorough coverage of all of the techniques used in the investigation of patients with blood disorders, including the latest technologies as well as traditional manual methods of measurement. You’ll find expert discussions of the principles of each test, possible causes of error, and the interpretation and clinical significance of the findings.
Collection and handling of blood
Reference ranges and normal values
Basic haematological techniques
Preparation and staining methods for blood and bone marrow films
Blood cell morphology in health and disease
Supplementary techniques including blood parasite diagnosis
Bone marrow biopsy
Molecular and cytogenetic analysis
Iron deficiency anaemia and iron overload
Investigation of megaloblastic anaemia: cobalamin, folate and metabolite status
Laboratory methods used in the investigation of the haemolytic anaemias
Investigation of the hereditary haemolytic anaemias: membrane and enzyme abnormalities
Acquired haemolytic anaemias
Investigation of variant haemoglobins and thalassaemias
Erythrocyte and leucocyte cytochemistry
Immunophenotyping by flow cytometry
Diagnostic radioisotopes in haematology
Investigation of haemostasis
Investigation of a thrombotic tendency
Laboratory control of anticoagulant, thrombolytic and antiplatelet therapy
Blood cell antigens and antibodies: erythrocytes, platelets and neutrophils
Laboratory aspects of blood transfusion
Approach to the diagnosis and classification of blood cell disorders
Laboratory organisation, management and safety
Quality assurance
Haematology in under-resourced laboratories