Li Shaoguang, Zhang Haojian (eds.) Chronic Myeloid Leukemia. Methods and Protocols

Springer; Humana Press, 2016. — xi, 257 p. — (Methods in Molecular Bilology 1465).Cancer stem cells are responsible for the initiation and progression of many types of cancer and are in general less sensitive to chemotherapy. There is an urgent need to fully understand the biology of cancer stem cells for developing effective therapies, and the success in this approach relies on utilizing stem cell disease models and advanced technologies to study the molecular basis of cancer stem cells. It is generally believed that human chronic myeloid leukemia (CML) is derived from an abnormal hematopoietic stem cell harboring Philadel- phia chromosome that results in the formation of the BCR-ABL oncogene. Leukemia stem cells (LSCs) in CML provide an ideal cellular system for studying how LSCs survive, proliferate, and evade from treatment with tyrosine kinase inhibitors. Technically, various molecular and cellular methods have been utilized to help understand the biology of LSCs in CML, and these methods have led to breakthrough discoveries useful in developing effective therapeutic strategies for CML. This book highlights the molecular and cellular methods used in studying CML pathogenesis and stem cell biology.