Dunn J.K. (ed.) Manual of diagnostic cytology of the dog and Cat

Wiley Blackwell, 2014. — 289 p. — ISBN: 978-1-118-78311-5.Wish you could interpret cytological specimens in practice rather than paying a lab to do it for you? Want to provide your clients with a faster service? Manual of Diagnostic Cytology of the Dog and Cat is the ideal quick reference for the busy veterinarian in first opinion practice. It describes techniques for obtaining good quality cytological diagnostic specimens, and guides you through the interpretation of cytological findings. Created to be used alongside the microscope, hundreds of high quality colour photos will help you to identify normal cell types and abnormal cytology, including both non-neoplastic and neoplastic lesions. It describes in a clear and concise manner the most common lesions and related disorders encountered in a practice setting. The concise format means that you can quickly find exactly what you're looking for. Covering indications for cytological investigation, collection techniques and the evaluation and interpretation of findings, this concise manual will be your go-to resource.Content: Manual of Diagnostic Cytology of the Dog and Cat1 Cytological Collection Techniques and Sample Preparation
Sampling techniques
Fine-needle aspiration
Impression smears/imprints
Scrapings
Swab smears
Brushings
Collection and handling of fluid samples for cytological examination
Staining techniques
Microscopic examination of cytological specimens
Recognition of artefacts
2 General Principles of Cytological Interpretation
Initial assessment of the sample: Is the preparation of diagnostic quality?
Inflammation. Neutrophilic inflammationMacrophage-rich or granulomatous inflammation
Eosinophilic inflammation
Lymphocytic/plasmacytic inflammation
Mixed inflammation
Aspiration of normal or hyperplastic tissue
Neoplasia
Criteria for malignancy
Dysplasia
Characteristics of cell populations: Epithelial, mesenchymal or discrete round cell?
Epithelial
Mesenchymal
Discrete round cells
Cystic structures
3 Cytology of the Lymphoid Tissues
The lymph node
Normal cytological appearance and benign changes
Non-lymphoid tissue
Reactive hyperplasia
Lymphadenitis
N eoplasia
Metastatic malignancies. Lymphoid malignanciesMorphology of canine lymphomas classified by the Kiel classification
Histiocytic sarcoma
The spleen
Benign changes
Neoplasia
Thymus
4 Cytology of Cutaneous and Subcutaneous LesionsNormal cell types
Non-neoplastic lesions
Neoplastic lesions
Epithelial tumours
Mesenchymal tumours
Round cell tumours
5 Cytology of the Respiratory TractCollection techniques
Nasal flush
Transtracheal wash and bronchoalveolar lavage
Transtracheal wash
Bronchoalveolar lavage
Transthoracic fine-needle aspiration
Cytological evaluation. Nasal cytologyNormal cell types
Inflammation/infection
Neoplasia
Cytology of the trachea and bronchial tree
Normal cell types
Mucus
Oropharyngeal contamination
Inflammation
Eosinophilic inflammation
Dysplastic epithelial cells
Squamous metaplasia
Intrapulmonary haemorrhage
Neoplasia (primary or metastatic)
6 Biochemical and Cytological Evaluation of Body Cavity EffusionsSample collection and processing
Classification of effusions
Traditional system
Aetiological system
Artefacts7 Cytology of Synovial Fluid
Introduction. CollectionRestraint
Asepsis
Equipment
Approach
Sample handling
Synovial fluid propertiesColour and turbidity
Viscosity
Mucin quality
Total nucleated cell count
Total protein
Cytology
Culture
Ancillary diagnostic tests
Antinuclear antibodies (ANA)
Rheumatoid factor
Classification of joint disease
Inflammatory arthropathies
Infectious arthritis
Non-infectious arthritis
Degenerative joint disease
Haemarthrosis
Neoplasia
8 Biochemical and Cytological Examination of Cerebrospinal Fluid
Contraindications
CSF collection sites and technique