Egelman E. (Ed.) Comprehensive Biophysics (Part I, Volumes 1-4)

Academic Press, 2012. - 1673 p. - Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics will help bridge that communication gap.
Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge.Key Features- Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding
- The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research
- Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resourceVolume 1 Biophysical Techniques for Structural Characterization of Macromolecules
Volume Introduction
Cantor and Schimmel – 30 Years Later
Efficient Strategies for Production of Eukaryotic Proteins
X-Ray Crystallography: Crystallization
X-Ray Crystallography: Data Collection Strategies and Resources
Phasing of X-ray Data
Refinement of X-ray Crystal Structures
Structure Validation and Analysis
Introduction to Solution State NMR Spectroscopy
Solid State NMR Methods
The Hybrid Solution/Solid-State NMR Method for Membrane Protein Structure Determination
Labeling Techniques
NMR Spectroscopy: NMR Relaxation Methods
Structure Determination of Macromolecular Complexes by Cryo-Electron Microscopy in vitro and in situ
Analysis of 2-D Crystals of Membrane Proteins by Electron Microscopy
Cryo-Electron Microscopy and Tomography of Virus Particles
Mass Spectrometry
Small and Wide Angle X-ray Scattering from Biological Macromolecules and their Complexes in Solution
Ultrafast Structural Dynamics of Biological Systems
Electron Magnetic Resonance
Computation of Structure, Dynamics, and Thermodynamics of Proteins
Rapid Mixing Techniques for the Study of Enzyme Catalysis
Optical Spectroscopy
Fluorescence and FRET: Theoretical Concepts 101Volume 2 Biophysical Techniques for Characterization of Cells
Volume Introduction
Elucidating Cellular Structures
Confocal Microscopy
Fluorescence Lifetime Microscopy: The Phasor Approach
Super-Resolution Microscopy
Studying the Macromolecular Machinery of Cells in situ by Cryo-Electron Tomography
Visualizing Sub-cellular Organization Using Soft X-ray Tomography
Atomic Force Microscopy
Super-Resolution Near-Field Optical Microscopy
CARS Microscopy
Elucidating Cellular Dynamics
Quantitative Fluorescent Speckle Microscopy
Fluorescence Correlation Spectroscopy
Image Correlation Spectroscopy
The Basics and Potential of Single-Molecule Tracking in Cellular BiophysicsVolume 3 The Folding of Proteins and Nucleic Acids
Protein Folding
Combining Simulation and Experiment to Map Protein Folding
Globular Proteins
Energetics of Protein Folding
Fast Events in Protein Folding
Intermediates in Protein Folding
Characterization of the Denatured State
Single-Molecule Spectroscopy of Protein Folding
Simulation Studies of Force-Induced Unfolding
Protein and Nucleic Acid Folding: Domain Swapping in Proteins
Intrinsically Disordered Proteins
Chaperones and Protein Folding
Protein Switches
Repeat/Non-Globular Proteins
The Folding of Repeat Proteins
Membrane Proteins
The Membrane Factor: Biophysical Studies of Alpha Helical Transmembrane Protein Folding
Nucleic Acid Folding
Effect of Protein Binding on RNA FoldingVolume 4 Molecular Motors and MotilityGeneral Theoretical Considerations
Microscopic Reversibility and Free-Energy Transduction by Molecular Motors and Pumps
Actin
Structure and Dynamic States of Actin Filaments
Actin Filament Nucleation and Elongation
Mechanical Properties of Actin Networks
Microtubles
Tubulin and Microtubule Structure: Mechanistic Insights into Dynamic Instability and Its Biological Relevance
Force Generation by Dynamic Microtubule Polymers
Myosin
Myosin Motors: Structural Aspects and Functionality
Myosin Motors: Kinetics of Myosin
Single Molecule Fluorescence Techniques for Myosin
Muscle
Cell-Based Studies of the Molecular Mechanism of Muscle Contraction
Spectroscopic Probes of Muscle Proteins
Thin Filament Regulation
Smooth Muscle and Myosin Regulation
Non-Muscle Motility
Intracellular Transport: Relating Single-Molecule Properties to In Vivo Function
Mechanical Forces in Mitosis
Kinesin
Kinesin Structure and Biochemistry
Kinesin Single-Molecule Mechanics
Dynein
Cytoplasmic Dynein: Its ATPase Cycle and ATPase-dependent Structural Changes
Axonemal Motility
The Ribosome
Viral DNA Packaging Motors