Walker J.M., Rapley R. (Eds.) Molecular Biomethods Handbook

Second edition. – Humana Press, 2008. – 1104 p.Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up-to-date collection of methods used regularly in the authors’ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.Part A Nucleic Acid Methods
Gayle Corkill and Ralph Rapley The Manipulation of Nucleic Acids: Basic Tools and Techniques
Gareth J. S. Jenkins Restriction Enzymes: Tools in Clinical Research
Bimal D. M. Theophilus Principles and Medical Applications of the Polymerase Chain Reaction
Marilena Aquino de Muro Probe Design, Production, and Applications
Bronwen M. Harvey and Pirkko Soundy Southern Blotting as a Diagnostic Method
Beatriz Sanchez-Vega Capillary Electrophoresis of DNA: Biomedical Applications
Kim Hung Leung and Shea Ping Yip Denaturing High-Performance Liquid Chromatography (DHPLC) for Nucleic Acid Analysis
Jeroen H. Roelfsema and Dorien J. M. Peters Denaturing Gradient Gel Electrophoresis (DGGE)
Kim Hung Leung and Shea Ping Yip Single Strand Conformation Polymorphism (SSCP) Analysis
Lúcia Maria da Cunha Galvão and Eliane Lages-Silva Randomly Amplified Polymorphic DNA (RAPD): A Useful Tool for Genomic Characterization of Different Organisms
David Sugden and Patricia de Winter Quantification of mRNA Using Real Time RT-PCR: The SYBR Solution
Anu Suomalainen and Ann-Christine Syvänen Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing
Carolina Sismani, Ludmila Kousoulidou, and Philippos C. Patsalis Multiplex Amplifiable Probe Hybridization (MAPH)
Arnis Druka, Robbie Waugh, and Pete Hedley Gene Expression Profiling
Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin Comparative Genomic Hybridization in Clinical and Medical Research
Craig Winstanley Subtractive Hybridization
Jane Bayani and Jeremy A. Squire Fluorescence In Situ Hybridization
Jonathan D. Smith Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice
Phillip G. Febbo cDNA Microarrays
Simon G. Gregory Mapping Techniques
Nameeta Shah Single Nucleotide Polymorphisms
Bernd H. A. Rehm and Frank Reinecke Gene/Protein Sequence Analysis: A Compilation of Bioinformatic Tool
Part B Protein and Cell Methods
David Sheehan and Siobhan O’Sullivan Protein Electrophoresis
Patricia Gravel Protein Blotting
Mark Strege Capillary Electrophoresis of Proteins
Bronwen M. Harvey Autoradiography and Fluorography
Kenneth G. Standing Mass Spectrometry of Proteins and Peptides
Christoph Kannicht and Birte Fuchs Post-Translational Modif ications of Proteins
Charlotte H. Clarke and Eric T. Fung Protein Microarray Technology
Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith D. Wilkinson, and Haian Fu Protein–Protein Interactions
Terry D. Butters and David C. A. Neville Glycoprotein Analysis
Mare Cudic and Gregg B. Fields Solid-Phase Peptide Synthesis
Zhong J. Zhang and Maher Albitar Monoclonal Antibodies
Rob Aitken Antibody Phage Display
Protein Engineering Thomas Willemsen, Urs B. Hagemann, Eva M. Jouaux,
Sabine C. Stebel, Jody M. Mason, Kristian M. Müller, and Katja M. Arndt
Sabine C. Stebel, Annette Gaida, Katja M. Arndt, and Kristian M. Müller Directed Protein Evolution
John Crowther Enzyme Linked Immunosorbent Assay (ELISA)
Glenn E. Morris Epitope Mapping: Identification of Antibody-Binding Sites on Protein Antigens
Charles Z. Hotz Quantum Dots
David Sheehan Ion-Exchange Chromatography
Paul Cutler Size-Exclusion Chromatography
Paul A. O’Farrell Hydrophobic Interaction Chromatography
Adam Charlton and Michael Zachariou Affinity Chromatography
Tzong-Hsien Lee and Marie-Isabel Aguilar High Performance Liquid Chromatography (HPLC) of Peptides and Proteins
Ian Davidson and Paula O’Connor Amino Acid Analysis
K. Scott Phillips and Quan Jason Cheng Surface Plasmon Resonance
Bernhard Rupp and Katherine A. Kantardjieff Macromolecular Crystallography
Anna C. Kinsella and Shelley D. Minteer Microchip Devices for Bioanalysis
Simon P. Langdon Mammalian Cell Culture
Víctor M. Loyola-Vargas, C. De-la-Peña, R. M. Galaz-Ávalos, and F. R. Quiroz-Figueroa Plant Tissue Culture
Mohan C. Vemuri and Chellu S. Chetty Stem Cells and Regenerative Medicine
John G. Day, Keith C. Harding, Jayanthi Nadarajan, and Erica E. Benson Cryopreservation: Conservation of Bioresources at Ultra Low Temperatures
Pottumarthi V. Prasad and Pippa Storey Magnetic Resonance Imaging
John Kuo Electron Microscopy
Guy Cox Confocal Microscopy
Graeme I. Murray Laser Microdissection
James L. Weaver and Maryalice Stetler-Stevenson Biomedical Uses of Flow Cytometry
Constance Oliver Immunomicroscopy
Ian A. Darby and Tim D. Hewitson In Situ Hybridization
William P. Janzen High Throughput Screening