Tseng F.-G., Santra T.S. (Eds.) Essentials of Single-Cell Analysis: Concepts, Applications and Future Prospects

Springer-Verlag Berlin Heidelberg, 2016. – 414 p. – ISBN10: 3662491168.Demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations.
This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis.TopicsBiomedical Engineering
Microengineering
Bioinformatics
Physics of the Cell
Cell BiologySingle-Cell Behavioral Assays for Heterogeneity Studies
Systems Biology in Single Cells
Electroporation for Single-Cell Analysis
Microinjection for Single-Cell Analysis
Optical Tools for Single-Cell Manipulation and Analysis
Optoelectrokinetic Manipulation for Cell Analysis
Continuous Micro-/Nanofluidic Devices for Single-Cell Analysis
Single-Cell Mechanical Properties: Label-Free Biomarkers for Cell Status Evaluation
Cytometry of Single Cells for Biology and Biomedicine
Single-Cell Genomics and Epigenomics
Single-Cell Metabolomics
Applications of Cell-Based Drug Delivery Systems: Use of Single Cell Assay
Applications of Single Cell Sequencing in Cancer
Single-Cell Characterization of Microalgal Lipid Contents with Confocal Raman Microscopy
Single Differentiated Neurons from Pluripotent Embryonic Stem Cells: Motor Protein Modeling and Neurodegenerative Disease