Schambra U. Prenatal mouse brain atlas

NY: Springer Science+Business Media, LLC, 2008. — 552 p. — ISBN: 978-0-387-47089-4. — e-ISBN: 978-0-387-47093-1.This atlas is designed to support research in neuroscience, gene manipulation, molecular biology and neurotoxicology. For researchers unfamiliar with the developing mouse brain, full sections are shown to aid in the orientation and comparison to their sections. This atlas provides complete sets of sagittal, coronal and horizontal sections, useful when determining which plane of sectioning may be most advantageous for a particular study. For instance, sagittal and horizontal sections are well-suited for a quick scan of a brain, while coronal sections provide more detailed information. In the color images the hematoxylin stain provides excellent information on neurons at different stages of development, i.e., neuroblasts in ventricular zones are darkly stained while maturing neurons in intermediate zones are lightly stained. In contrast, developing pathways and their glial scaffolding are stained deeply pink with eosin. Regions of particular developmental interest, for example the eye, cranial nerve nuclei, or ganglia, are photographed at higher power to show different stages of development. Special attention has been given to ventricular zones, the origin of all brain structures, to assist tracking cells carrying a specific gene. For instance, using the bacterial artificial chromosome vector method of producing transgenic mice (Nature 425: 917-925, 2003), the development of the neurons in a gene specific nucleus coul be traced back to two progenitor cell in a related ventricular zone.Color images and annotated diagrams of: Gestational days 12, 14, 16 and 18; sagittal, coronal and horizontal section.Preface

Methods

Gestational day 12
16 sagittal sections
22 coronal sections
18 horizontal sections
Gestational day 14
20 sagittal sections
33 coronal sections
24 horizontal sections
Gestational day 16
16 sagittal sections
30 coronal sections
17 horizontal sections
Gestational day 18
17 sagittal sections
26 coronal sections
15 horizontal sections