Humana Press. 1998. 326 p.
An unprecedented collection of core PCR techniques useful for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells. The book demonstrates over and over again the power of PCR-its high sensitivity, specificity, and ability to rapidly discriminate sequence variations.
Introduction to the Polymerase Chain Reaction
Setting Up a PCR Laboratory
Amplification from Archival Materials
Quantitative PCR
Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols
Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal Labeling
The Amplification Refractory Mutation System
Artificial Restriction Fragment Length Polymorphism (A-RFLP) Analysis
Long Range PCR
Generation of Labeled Probes by PCR
PCR for the Detection of Minority DNA Populations
In Situ Amplification
Sequencing of PCR Products
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation
Diagnosis of Mitochondrial Disorders Using the PCR
PCR Analysis of CD44 Variants in Tumors
Detection of Circulating Solid Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction
Detection of Circulating Tumor Cells Using Immunobead-PCR
Single Cell PCR: Theory, Practice, and Clinical Applications
PCR-Based Noninvasive Prenatal Diagnosis Using Fetal Cells in Maternal Circulation
Noninvasive Prenatal Diagnosis Using a Single Fetal Nucleated Erythrocyte Isolated by Micromanipulation from Maternal Blood
Diagnosis of Chromosomal Aneuploidies Using Quantitative Fluorescent PCR
Detection of the Hepatitis C Virus by RT-PCR
The Application of PCR to the Detection of M. tuberculosis in Sputum Samples
Microsatellite Analysis in Human Disease